echinus, required for interommatidial cell sorting and cell death in the Drosophila pupal retina, encodes a protein with homology to ubiquitin-specific proteases

Table 2 Bacterial Assay for the Deubiquitinating Activity of Echinus

Table 2. Echinus ec-SF1 lacks deubiquitinating activity on a model substrate in bacteria. Ubiquitin-Arg-β-Gal has a long half-life, and colonies expressing this protein alone are therefore blue in the presence of X-gal substrate. Expression of S. cerevisiae Ubp2 with Arg-β-Gal results in cleavage of ubiquitin, exposing the N-terminus of Arg-β-Gal, which has a short half-life (white colonies). In contrast, expression of ec-SF1 or ec-SF2 does not result in significant cleave Ub-Arg-β-Gal (blue colony color).

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