Table 3 Primer Sequences for Q-PCR
Gene | Primer | Sequence | Gene | Primer | Sequence |
|---|---|---|---|---|---|
Gata6 | F | GAGCTGGTGCTACCAAGAGG | Plat | F | GATGACAGGGAGATGCCAAC |
| Â | R | TGCAAAAGCCCATCTCTTCT | Â | R | CTTGTCCCCAGTGCAAACTT |
Gata4 | F | CCCTTCCCTCTTCAAATTCC | mSna | F | GCTGTGTTGGAAACGGAGTT |
| Â | R | CTTTTCCAGAGCTCCACCTG | Â | R | CATGTGGGTTCTGACTGGTG |
Sox7 | F | GCTCCTGCTTTTGGTGTAGC | SPARC | F | GTTCCTGCTTGGCTCTCTTG |
| Â | R | GTCCTTGGGCAGTCATTCAT | Â | R | CCTTGAGGGAGGTAGGGAAG |
Sox17 | F | GAGGGCCAGAAGCAGTGTTA | Follistatin | F | ACCTGAGAAAGGCCACCTG |
| Â | R | AGTGATTGTGGGGAGCAAGT | Â | R | AGCTTCCTTCATGGCACACT |
Dab2 | F | TCTCAGCCTGCATCTTCTGA | Pthr1 | F | AGGACGACGGCTTCCTTAAT |
| Â | R | GAGCGAGGACAGAGGTCAAC | Â | R | TTGTCTTCCTGGTCCAGTCC |
AFP | F | TCCAGAAGGAAGAGTGGACAA | Oct3/4 | F | CACGAGTGGAAAGCAACTCA |
| Â | R | GCAGACTAGGAGAAGAGAAATAGTTGA | Â | R | AGATGGTGGTCTGGCTGAAC |
ApoE | F | GGTTCGAGCCAATAGTGGAA | Nanog | F | CACCCACCCATGCTAGTCTT |
| Â | R | TATTAAGCAAGGGCCACCAG | Â | R | ACCCTCAAACTCCTGGTCCT |
RBP | F | GAACTTCGACAAGGCTCGTTTCTCGG | Rex1 | F | GAGTTCGTCCATCTAAAAAGGGAGG |
| Â | R | ATCCAGTGGTCATCGTTTCCTCGCT | Â | R | TCTTAGCTGCTTCCTTGAACAATGCC |
Ihh | F | GGCCTGGGATTGTGACTTTA | Cdx2 | F | AGGCTGAGCCATGAGGAGTA |
| Â | R | CTGCAGGGAAGGTCATGTTT | Â | R | CGAGGTCCATAATTCCACTCA |
Hnf3b | F | CCCTGCTAGCTCTGGTCACT | Hand1 | F | CCCCTCTTCCGTCCTCTTAC |
| Â | R | ACAGATCACTGTGGCCCATC | Â | R | CTGCGAGTGGTCACACTGAT |
Msg2 | F | ATGCCAACCAGGAGATGAAC | Psx1 | F | GAATTGGTTTCGGATGAGGA |
| Â | R | AGGATGCAGGTTGAAGGATG | Â | R | GTGGCTCAGAAGAAGCCATC |
Ttr | F | CTCACCACAGATGAGAAG | Gapdh | F | ACCACAGTCCATGCCATCAC |
| Â | R | GGCTGAGTCTCTCAATTC | Â | R | TCCACCACCCTGTTGCTGTA |