Figure 6

Knock-down of Gata6 in late-passage gExEn cells. (A-C) Efficient transfection of g6ExEn cells by EGFP expression vector. After drug selection, almost all transfectants showed GFP expression microscopically (A: phase-contrast image, B: fluorescent image for EGFP), which was confirmed by FACS analysis for EGFP fluorescence (C). (D, E) Morphology of g6ExEn cell line, 1D3, transfected with the Gata6 silencing vector pSil-G6 (D) or empty vector (D) after 5 days transfection. (F) Expression level of Gata6 and Afp in Gata6-silenced 1D3 cells by Q-PCR at day 5. Results were normalized relative to expression of Gapdh and plotted relative to expression level in 1D3 cells transfected with pSil-H1puro.