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Figure 5 | BMC Developmental Biology

Figure 5

From: Extra-embryonic endoderm cells derived from ES cells induced by GATA Factors acquire the character of XEN cells

Figure 5

Expression of endogenous Gata4 and Gata6 in late-passage gExEn cells. (A) Marker gene expression in late passage: g6ExEn cells expressed endogenous Gata4 and Gata6 slightly higher than in early passage g6ExEn cells. All results were normalized relative to expression of Gapdh and plotted relative to the expression level in 5G6GR-derived PrE without LIF for 5 days. (B) After removal of Gata6-GR activity by withdrawal of Dex from passage10 to passage20 (P10-P20), the expression levels of endogenous Gata4 and Gata6 were maintained as same amount level in the presence of Dex culture condition (P20). All results were normalized relative to expression of Gapdh and plotted relative to the expression level in P20 g6ExEn cells.

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