Figure 4

Effect of the extinction of exogenous GATA activity in gExEn cells. (A-D) Photomicrographs of g6ExEn11-2 (A, B) and g4ExEn4-3 (C, D) cells cultured with (A or C) or without (B or D) Dex for 4 days after 2 passages in the presence of Dex. (E, F) Q-PCR analysis of PE or VE marker gene expression in g6ExEn11-2 cells, with or without Dex. Withdrawal of Dex after 2 passages in the presence of Dex decreased expression of a set of PE marker genes (E), while expression of VE marker genes increased in parallel (F). All results were normalized relative to expression of Gapdh and plotted relative to the expression level in Dex-treated g6ExEn11-2 cells.