Figure 2

Marker gene expression of gExEn cells. (A-E) Q-PCR analysis of gene expression in Dex-treated gExEn cells after 10 passages. ExEn markers (A), PE markers (B and C), VE markers (D) and stem cell markers (E). Relative expression levels of the indicated marker genes in two independent clones of g6ExEn (2-3 and 11-2) and g4ExEn (1-1 and 4-3) and in 5G6GR ES cells, in the presence and absence of LIF for 5 days, are shown. All results were normalized relative to the level of expression Gapdh and plotted relative to expression levels in 5G6GR-derived PrE without LIF (A-D) or that in 5G6GR ES cells (E). (F) g4ExEn4-3 were stained with anti-GATA6 (red), anti-DAB2 (green), and nuclear staining by Hoechst33342 (blue). The lower right panel shows marginal images.