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Table 2 Assets and limitation of in situ electroporation and retroviral transduction to target YS blood islands precursors

From: Gene transfer to pre-hematopoietic and committed hematopoietic precursors in the early mouse Yolk Sac: a comparative study between in situ electroporation and retroviral transduction

 

In situ electroporation

Retroviral transduction

 

Asset

Limitation

Asset

Limitation

7–7.5 dpc: Mesodermal/pre-hematopoietic precursors

- Transient

- Layer specific delivery

- Spatial control of delivery

- Reduced number of precursors available for transduction

 

- Causes extensive cell death

- Refractory to transduction ?

Application

Only possible method

Unsuitable at this development stage

From 7.5–8 dpc: Hematopoietic precursors

- Transient

- Layer specific delivery

- Spatial control of delivery

- Only a fraction of the YS blood islands is targeted

- Easy

- All hematopoietic precursors may be transduced

- Does not allow layer specific targeting

Application

-Transient transgene delivery

- Sustained expression of the transgene

 

- Possible layer specific delivery.

- Possible delivery of ShRNA

 

- Possible delivery of SiRNA or proteins

  
  1. Hematopoietic precursor frequency within whole control YS (electroporated without construct) and GFP+ cells sorted from electroporated YS-explants were determined through limiting dilution assay.