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Table 1 In situelectroporation efficiently delivers the construct to the blood islands

From: Gene transfer to pre-hematopoietic and committed hematopoietic precursors in the early mouse Yolk Sac: a comparative study between in situ electroporation and retroviral transduction

 

Frequency of recovered hematopoietic precursors

Number of recovered hematopoietic precursors per YS

Number of experiments

Whole OrgD3-YS

1/475

10–11

4

Whole OrgD3-YS electroporated without plasmid

1/453

10

3

OrgD3-YS GFP + cells

1/150 (± 57.62)

0.56 ± 0.18 per 0.5 YS (targeted area)

5

  1. Hematopoietic precursor frequency within whole control YS (electroporated without construct) and GFP+ cells sorted from electroporated YS-explants were determined through limiting dilution assay.
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BMC Developmental Biology

ISSN: 1471-213X

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