Figure 7

Hematopoietic development from transduced cells. A: Comparison of the clonogenic potential of GFP⁺ cells sorted at day 4 post-infection from YS infected upon explantation or after one day in organ culture. B: Phenotype analyses of OrgD1-YS infected cells cultured for 1 day on OP9 stromal cells, show that erythroid (Ter199⁺) and myeloid (CD45⁺) cells are identically distributed within GFP⁺ and GFP^- subsets. C: At day 4 post infection, the distribution of erythroid (Ter199⁺) and myeloid (CD45⁺) cells within the GFP⁺ population is similar to that obtained from normal YS (never exposed to viral supernatant). D: Clonogenic assays, performed at day 1 (left panel) and 4 (right panel) post-infection, indicate that the transduction procedure does not modify the type and numbers of precursors recovered.