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Figure 1 | BMC Developmental Biology

Figure 1

From: Gene transfer to pre-hematopoietic and committed hematopoietic precursors in the early mouse Yolk Sac: a comparative study between in situ electroporation and retroviral transduction

Figure 1

Determination of the development stages enriched in immature precursors. Upper panel: Temporal evolution of lmo2 expression from the OB to LHF stages: initially expressed by most extra-embryonic mesodermal cells (A), lmo2 expression rapidly restricts to a smaller cell number at the EB stage (B). Lmo2 expression subsequently expands with the development of blood islands endothelial and hematopoietic cells from LB (C) to LHF (D), and subsequent stages. Middle panel: Transcripts of the embryonic globin β-H1 are present in a minute cell number at the OB/EB stages (E, F). During the following stages (LB: G to LHF: H, and subsequent stages), β-H1 expressing erythroid cells rapidly expand. The arrows in the upper and middle panels point to equivalent zone of the blood islands. Lower panel: The comparative evolution of lmo2 and β-H1 expressions points to embryos at the OB/EB stages as enriched in immature precursors. Scale bar: 100 μm.

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BMC Developmental Biology

ISSN: 1471-213X

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