Figure 7
From: Requirement of histone deacetylase activity for the expression of critical photoreceptor genes
Effect of HDAC inhibition on the development of retinal cell types. P2 mouse retinal explants were cultured in the presence of 100 nM TSA or DMSO. After 8 days in culture, cells were dissociated and immunostained with cell type-specific antibodies. Marker positive cells were counted for each antibody from 3 independent experiments. Eight hundred to 1000 cells were counted for each antibody in each experiment. In all of the three experiments, not a single cell was detected as Rhodopsin or GS positive when retinal explants were treated with TSA. Significance analysis was performed using a Student's t-Test. *** p < 0.05. (BP-bipolar cells, Prog-progenitor cells, RBP-rod bipolar cells, AC-amacrine cells, HC-horizontal cells, GC-ganglion cells, MG- Müller glial cells.)