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Figure 2 | BMC Developmental Biology

Figure 2

From: Requirement of histone deacetylase activity for the expression of critical photoreceptor genes

Figure 2

Effects of HDAC inhibition on retinal gene expression. P2 mouse explant cultures were treated with 1 μM TSA or DMSO vehicle control for 3 hours. Total RNA was extracted and labeled with cy3 or cy5 for TSA and DMSO treatments, respectively. cDNA microarray analysis is shown by a scatter plot in (A). TSA-downregulated genes are distributed above the 45° diagonal; and TSA-upregulated genes are distributed below it. TSA-downregulated genes listed in (B) encompass photoreceptor genes (Otx2 and Crx) and proneural genes (NeuroD1 and Math3/NeuroD4) as well as cell cycle regulators (CyclinD1, Cdk4, and Cdk2). TSA-upregulated genes shown in (C) include a synaptosomal-associated protein, SNAP25. Values given (B and C) represent the fold change upon TSA treatment. The second value (cy5/cy3) shows the fold change in a dye swap experiment.

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