Figure 8

Map of P2Y ₁ receptor protein in neonatal versus juvenile rat cerebellar cortex. Double immunofluorescence visualized by confocal analysis was performed in neonatal rat (P7) cerebellar cortex and compared to that at P21 (inset in panel A). At P7, P2Y₁ receptor immunoreactivity (red Cy3 immunofluorescence) is present on calbindin-positive pc (green Cy2 immunofluorescence), on fibers irradiating from the thin ml toward the egl (A, B), on cell bodies at the interface between the pl and ml (black arrow in the left inset of panel B), and on isolated cells situated in the igl (white arrows in panel B). Double immunofluorescence (yellow) shows complete colocalization between P2Y₁ (red Cy3 immunofluorescence) and GFAP (green Cy2 immunofluorescence) signals (white arrows in B; black and white arrows in the upper-left inset of panel B), differently from what observed at P21 (lower-right inset of panel B). In C, P2Y₁ (red Cy3 immunofluorescence) and NFL (green Cy2 immunofluorescence) immunostaining are shown. Abbreviations: egl, external granule layer; gl, granule layer; igl, internal granule layer; ml, molecular layer; pc, Purkinje cells. Scale bar = 20 μm in A, B and C; 50 μm in the inset in A; 10 μm in the upper-left inset in B; 40 μm in the lower-right inset in B.