Figure 4

Beaded appearance of P2Y ₁ receptor immunostaining. Sagital sections of P21 rat cerebellar cortex were processed for triple immunofluorescence analysis and visualized by confocal microscopy. Rabbit anti-P2Y₁ (red Cy3 immunofluorescence) was used in combination with mouse anti-synaptophysin, a marker for synaptic structures (green Cy2 immunofluorescence), and mouse anti-flotillin, a lipid rafts-associated integral membrane protein (blue immunofluorescence). In gl, the overlapping immunoreactive signals of P2Y₁, synaptophysin and flotillin (arrows) can be distinguished by white immunofluorescence (arrows). Scale bars: 10 μm and 20 μm in the insets.