Figure 3
From: P2Y1receptor switches to neurons from glia in juvenile versus neonatal rat cerebellar cortex
Localization of P2Y 1 receptor on DβH neurons. Confocal images demonstrate that P2Y1 receptor immunoreactivity (red Cy3 immunofluorescence) colocalizes with DβH, specific marker of noradrenergic neurons (green Cy2 immunofluorescence). In panel B, a higher magnification of sparse and unbundled noradrenergic fibers of different thickness (arrows) and beaded nature (arrow head and inset) is shown. Panel C represents a triple immunofluorescence performed with anti-P2Y1 receptor (red Cy3 immunofluorescence), anti-DβH (green Cy2 immunofluorescence), and anti-Calbindin-D-28K (blue immunofluorescence) antibodies. Scale bars are: 20 μm in A and B, and 5 μm in C and in the inset in B.