Table 2 Identification of putative genes from SSH of tester cDNA from metamorphically committed larvae and driver cDNA from feeding 5th instar larvae
Classification | No | Tissue sources | Similar genes in GenBank | E-value | Functions of the genes |
|---|---|---|---|---|---|
Ribosome proteins | 1 | epidermis | ribosome protein S23 [D.melanogaster] | 4e-06 | see Table 1 |
| Â | 2 | epidermis Fat body midgut head | ribosome protein L27 [S. frugiperda] | 4e-54 | see Table 1 |
| Â | 3 | fat body head | ribosome protein S7 [B.mori] | 7e-24 | see Table 1 |
| Â | 4 | epidermis midgut head | ribosome protein L13 [Helicoverpa zea] | 2e-24 | see Table 1 |
| Â | 5 | head | ribosome protein L9 [B. mori] | 2e-26 | compose the large ribosomal subunit |
| Â | 6 | head | ribosome protein L11 [B. mori] | 5e-22 | large ribosomal subunit protein |
| Â | 7 | head | ribosomal protein L41 [S. frugiperda] (BLAST by nucleotide acids) | 1e-23 | mitochondrial ribosomal protein |
Hydrolases | 8 | fat body | DEAD box RNA helicase [C. fumiferana] | 1e-25 | disrupt RNA-protein interactions |
| Â | 9 | midgut | ribonuclease [Ceratitis capitata] | 8e-31 | degradation of RNA |
Immune-related proteins | 10 | midgut | immune inducible protein [B. mori] | 3e-11 | immune-response |
| Â | 11 | midgut | lysozyme [Heliothis virescens] | 1e-17 | immune |
Regulator | 12 | epidermis | basic juvenile hormone-suppressible protein 2 precursor (BJHSP2) [S. litura] | 6e-08 | metamorphosis related |
| Â | 13 | epidermis | promoting protein [B. mori] | 3e-12 | promoting in vitro replication of nucleopolyhedrovirus |
| Â | 14 | head | basic leucine zipper and W2 domains 2 [R. norvegicus] | 0.009 | transcription factor controlling molting and metamorphosis |
| Â | 15 | fat body | guanine nucleotide binding protein gamma subunit-like protein [P. xylostella] | 4e-22 | Signal transduction |
| Â | 16 | midgut | conserved hypothetical protein [Lactococcus lactis] | 5e-19 | - |
Enzyme | 17 | fat body | ATP synthase subunit 6 [Phthonandria atrilineata] | 0.15 | ATP synthase |
| Â | 18 | midgut | luciferase [Luciola lateralis] | 8e-13 | Fatty acyl-CoA synthetase |
| Â | 19 | head | IMP dehydrogenase/GMP reductase [Desulfuromonas acetoxidans DSM 684] | 0.31 | re-utilization of free intracellular bases and purine nucleosides |
Transporter | 20 | epidermis | cation channel family protein [Tetrahymena thermophila SB210] | 6.1 | Ca2+-permeable ion channel |
| Â | 21 | midgut | nuclear transport factor 2 [Aedes aegypti] | 3e-24 | targeting proteins into the nucleus |
Others | 22 | midgut | phenoloxidase inhibitor protein [Anopheles gambiae] | 0.006 | enzyme inhibitor |
| Â | 23 | epidermis | apolipophorin-III precursor [Agrius convolvuli] | 1e-12 | lipid transfer and cell death |
| Â | 24 | midgut | CG12120-PA [Tribolium castaneum] | 2e-09 | - |
| Â | 25 | epidermis | hexamerine [H. armigera] | 2e-04 | storage protein during metamorphosis |
| Â | 26 | midgut | cobatoxin long form B [S. frugiperda] | 8e-05 | - |
| Â | 27 | midgut | sensory neuron membrane protein 2 [M. sexta] | 4e-05 | - |
| Â | 28 | head | 60 S ribosome subunit biogenesis protein [Theileria annulata] | 9.7 | - |
| Â | 29 | midgut | ENSANGP00000017373 [Apis mellifera] | 3e-10 | - |
| Â | 30 | head | CG12926-PA [T. castaneum] | 3e-19 | - |
| Â | 31 | midgut | deoxyribodipyrimidine photolyase [Marinobacter aquaeolei VT8] | 6.1 | - |
Unknown ESTs | 32–47 | midgut epidermis fat body head |  |  |  |