Table 1 Frequency of activation of in vitro maturing oocytes which were fertilised, during maturation, by ISCI, 1 – 4 h after GVBD, refertilized at metaphase II (ICSI + IVF) and were fixed for cytological examination 7 – 8 h after refertilisation. Control, in vitro maturing oocytes were inseminated once: only by ICSI during maturation (ICSI), only in MII stage (IVF) or were not inseminated at all (no ICSI and no IVF).

From: Mouse oocytes fertilised by ICSI during in vitro maturation retain the ability to be activated after refertilisation in metaphase II and can generate Ca²⁺oscillations