Figure 3

Halibut TnT genes tissue specific expression; (A) Northern blot analysis of the expression of halibut fTnThh, AfTnThh and sTnT2hh genes in adult white muscle (W), red muscle (R), heart (H) and liver (L). All images represent 48 hour exposures. Ethidium bromide gel image is shown to give an indication of the quantity of total RNA loaded per sample. (B) RT-PCR analysis of expression of fTnThh, AfTnThh and sTnT2hh in halibut adult white (WM) and red (RM) muscle and heart (H). The 18s rRNA is shown and was used for normalisation. The graphs on the right of the fTnThh and AfTnThh gels represent, respectively, expression of the different fTnThh or AfTnThh isoforms relative to 18s rRNA.