Functional trends of DEGs classified according to the Biological Process terms defined by GO consortium. (A) Developing workers up-regulate more developmental genes than queens in all studied larval instars. Physiometabolic genes are always more up-regulated than developmental genes (B) Juvenile hormone (JH) treatment induces a queen-like gene expression profile. Left panel: up-regulated genes in L4 queens/workers. Right panel: up-regulated genes in L4 Control/JH-treated workers. The proportion of Physiometabolic and Localization genes is higher in normal queens and JH-treated workers, whereas more Developmental genes are up-regulated in normal and in Control workers.