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Figure 1 | BMC Developmental Biology

Figure 1

From: Specific expression of lacZ and cre recombinase in fetal thymic epithelial cells by multiplex gene targeting at the Foxn1 locus

Figure 1

Multiplex gene targeting at the Foxn1 locus. A. Map of the wild-type (wt) Foxn1 locus and the targeting vectors used for gene targeting. Locations of the 5' and 3' flanking probes (fp) are shown. Exons 7–9 are shown as boxes, coding regions are black and UTR sequences are grey. IRES-Cre or IRES-lacZ cassettes including the floxed or frt neo positive selection cassette were inserted into a unique XhoI site (X) in the 3'UTR in exon 9. The targeting vector homology was flanked by two TKgenes for negative selection. B. Southern blot analysis of ES cell clones. Genomic DNA was digested with EcoRV, and probes with the 5' flanking probe. The wild-type 14.8 kb band is present in all samples. Correctly targeted cell lines with bands corresponding to the two different targeted alleles are present in lanes 3–6. Lane two shows an abberant clone. C. PCR analysis of the lacZ alleles used for genotypeing. The larger, 600 bp band is the wild-type allele, the 300 bp band is the lacZ (z) allele. Marker (M) shown is the Promega 1 kb ladder. V, EcoRV; E, EcoRI; B, BamHI; K, KpnI; S, SspI; X, XhoI.

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