Figure 8
From: Ovarian gene expression in the absence of FIGLA, an oocyte-specific transcription factor
Expression of FIGLA targets. Tissue-specific and developmental expression of eight genes potentially up-regulated by FIGLA in SAGE analysis. SAGE tags for each gene were absent in the Figla null newborn library and present ≥ 10 in the normal newborn library. A. Semi-quantitative RT-PCR of total RNA isolated from somatic and reproductive tract tissues with primers specific for each of the eight genes. B. qRT-PCR of total ovarian RNA isolated from normal and Figla null mice at E12.5, E13.5, E15.5, E17.5, E19.5, newborn (NB), 2dpp and 7dpp and plotted relative to HPRT. Data is the average (± s.e.m.) of 2–6 independently isolated biological samples, analyzed in triplicate at each developmental time point.