Figure 6

bHLH genes are inversely regulated by Ngn3 and Notch1^ICD. (A-T). In situ hybridization on serial sections from embryonic pancreata 52 hours after electroporation with control vector (A-E), Ngn3 (F-J), Notch1^ICD (K-O), or Ngn3 and Notch1^ICD (P-T). The expression patterns of Hes1 (B, G, L, Q)), Hes5-1 (C, H, M, R), Hes6-1 (D, I, N, S), and NeuroD (E, J, O, T) were examined. A, F, K, P show GFP expressing electroporated cells (arrowheads) and the corresponding area is indicated with arrowheads in the rest of the panels. Note that Notch1^ICD apparently leads to decreased Hes1 expression (red arrowhead in L and arrowheads in Q) and induces Hes5-1 expression (M, R) and repress Hes6-1 (N, S) and NeuroD (O, T) expression regardless of the presence of Ngn3.