Figure 2

Inhibition of Notch signaling induce excess endocrinedifferentiation. (A-D). Confocal sections of chicken embryonic pancreata 72 hours after electroporation with control vector (A), Delta1 (B), Serrate1 (C), and Serrate1-d1 (D) subjected to immunohistochemical stainings against GFP (green) and glucagon plus insulin (red). Cells expressing GFP from the vector readily co-localize with endocrine markers. However, most electroporated cells are not endocrine (A). In contrast, electroporation with either Delta1, Serrate1, or Serrate-d1 results in increased endocrine differentiation of electroporated cells as compared to the control (compare B, C, D to A). E. Quantitative analysis of endocrine differentiation 72 hours after electroporation with the indicated expression plasmids at HH st. 10–12 or HH st. 13–15. (F, G).