Figure 5
From: Generation of FGF reporter transgenic zebrafish and their utility in chemical screens
Validation of Tg(Dusp6:d2EGFP)pt6line for chemical screening. (A-Y) Lateral views of 30 hpf embryos treated with the compound and dose are indicated on the bottom right. The protein targets for these compounds are listed in the top right hand corner. (A) Embryo incubated in 0.5% DMSO as control. (B-D) Increasing doses of SU5402 suppressed d2EGFP expression in the MHB (red bracket), trigeminal ganglia (red arrow) and dorsal retina (red arrowhead). (E-H) Increasing doses of SU5416, a non-specific inhibitor of VEGFRs, suppressed FGF signalling. (I & J) Oxindole I another related VEGFR inhibitor also suppressed d2EGFP fluorescence in transgenic embryos. (K-N) In contrast, two compounds with similar chemical structure SU4312 and SU11652, did not block FGF signalling. (O & P) Likewise, two unrelated inhibitors of PDGFR and VEGFR, SU1433 and SU1498 also failed to suppress d2EGFP expression. (Q-T) PP2 and SU6656, two Src Kinase inhibitors suppressed FGF signalling in transgenic embryos.