The twisted pharynx phenotype in C. elegans

Table 1 Degrees of twist within the isthmus in animals of various ages and genotypes.

Genotype, Stage	Twist° in Individuals	Avg + SEM of \|Twist\|
Samples quantitated from 3D reconstructions
etIs2, Adult (1d)	17, 6, 23, 36	21 ± 6
etIs2;mnm-4, L1	54, 36, 45	45 ± 5
etIs2;mnm-4, L2	90, 72, 90, 90	86 ± 5
etIs2;mnm-4, L3	135, 107, 152, 163	139 ± 12
etIs2;mnm-4, L4	216, 190, 198, 198, 201	201 ± 4
etIs2;mnm-4, Adult (1d)	180, 198, 198, 198	194 ± 5
etIs2;mnm-4, Adult (6d)	180, 216, 144, 216, 189	189 ± 13
etEx2;mnm-4, Adult	220, 210, 186, 231	212 ± 10
etIs2;dig-1, Adult	+40, 169, +242, +163	154 ± 42
etEx2;dig-1, Adult	170, +90, 45, 62	102 ± 32
etIs2;unc-61, Adult	135, 129, 79, 90	114 ± 15
etEx3;mnm-4 unc-61, Adult	129, 146, 146, 141	141 ± 4
Samples quantitated from photographs
etIs2	0, 0, 0, 0	0
etIs2;mnm-4, L4	229, 264, 210	234 ± 16
mnm-4, L4	220, 169, 244	211 ± 22
etIs2/+;mnm-4/+, L4	79, 71, 71	74 ± 3
*mig-4, Adult	+106, +57, +166, 114, 95, 92	99 ± 14
mig-4;mnm-4, Adult	159, 172, 130	154 ± 12
*dig-1, Adult	+107, +46, +107, 108, 141	105 ± 15
dig-1;mnm-4, Adult	209, 109, 214	177 ± 34
eat-3;mnm-4, L4	119, 89, 123, 89, 126, 130, 85	109 ± 8
etIs2;mnm-4, early L2	0, 67, 90, 105	66 ± 23
Samples quantitated from photographs of the same four developing individuals
etIs2;mnm-4, late L2	131, 162, 150, 67	128 ± 21
etIs2;mnm-4, early L3	214, 157, 173, 144	172 ± 15
etIs2;mnm-4, late L3	217, 203, 140, 183	186 ± 17
etIs2;mnm-4, early L4	203, 186, 187, 204	195 ± 5

Twisting was measured from 3D reconstructions of worms expressing pRIC-19::GFP in their M2 neurons, or by applying the equation shown in Fig 1A on photographs. Besides the pRIC-19::GFP plasmid, the etIs2 integrated array and the etEx2 extrachromosomal array also carry the rol-6 transformation marker while the etEx2 extrachromosomal array carries the dpy-20(+) marker (see Materials and Methods). Most twisting pharynges twisted to the left; right-handed twists are indicated with plus signs ("+"). The dig-1 and mig-4 worms fell into two groups: those with no pharyngeal twist, and those with an obvious twist. 19 of 24 dig-1 and 18 of 24 mig-4 adult animals scored did not show any pharyngeal twisting. *Only those dig-1 and mig-4 worms with a pharyngeal were used to calculate the average twist of these mutants.

ISSN: 1471-213X