Figure 2
Description of isolated pharynges and abnormal pharynges. For panels A-F, pharynges were dissected from worms held on dried agarose pads (2% in H2O) covered with mineral oil and using a tungsten (0.5 mm) dissecting needle, then transferred to a drop of M9 on an agarose pad (2% in M9), allowed to relax by swirling the liquid before applying a coverslip, and examined under the microscope. Note that the control pharynx of wild-type worms carrying the etIs2 integrated sequence is straight, and that the isolated pharynges of mnm-4;etIs2, mnm-4, mig-4 and unc-61 worms are twisted whereas the pharynx of the dig-1 animal is untwisted. Panels G-J show the pharyngeal twist in mnm-4 animals carrying mutations that cause abnormal pharynx morphologies (pha-2, sma-1) or reduced pumping rate (eat-3). Worms in G and H are adults, and L4 larvae are shown in I-J. Note that the enlarged procorpus of mnm-4;sma-1 worms and the enlarged isthmus of mnm-4; pha-2 worms show less twisting then the corresponding part of control worms, and that the mnm-4 is slightly more twisted than the eat-3;mnm-4 worm.