Table 4 List of the primer pairs for quantitative RT-PCR. Thirteen genes were selected as being up-regulated in Hamburg and Hamilton (HH) stage 3 embryo from microarray data.
From: A set of stage-specific gene transcripts identified in EK stage X and HH stage 3 chick embryos
# | GeneChip Probe Set ID | Gene Title | Forward | Reverse |
|---|---|---|---|---|
1 | GgaAffx.21413.1.S1_s_at | Finished cDNA, clone ChEST629c13 | cccagtcccagtgtttgagt | acaagcgatgaaagaaagcc |
2 | Gga.7813.1.S1_at | Finished cDNA, clone ChEST148d1 | cagcacacagaaatgggaga | ctgaagaagcacacagcagg |
3 | Gga.8314.1.S1_at | Transcribed locus | tgtcataaacggggaaaagtg | gctgaaagagactgtgataaaccatac |
4 | Gga.12166.1.S1_at | zinc finger homeobox 1b | aagtcgagtgcaattcgtaca | tggactaaaggggcagttca |
5 | Gga.19405.1.S1_at | Finished cDNA, clone ChEST666e20 | tgctctgtccttcttctgcc | ccccaaccatctctccagt |
6 | GgaAffx.9643.1.S1_at | proprotein convertase PC6 | tcttcttcaactgcctgcct | gaacaccgtccttcgtcact |
7 | Gga.16092.1.S1_at | Finished cDNA, clone ChEST357i21 | ttggcaggaaggaagacaag | gcaactctacgaactggctg |
8 | GgaAffx.21109.1.S1_s_at | Finished cDNA, clone ChEST172i5 | ggttggaatcttgtggcagt | gcttgttggtcggtcttctc |
9 | Gga.13785.1.S1_at | heterogeneous nuclear ribonucleoprotein L-like | gctttcatcaggttaatgttgc | catgctaatgcaaacggaaa |
10 | Gga.18924.1.S1_at | Finished cDNA, clone ChEST628k5 | accacaggcagtacggctac | ccccaaggaaaatttaaaacg |
11 | Gga.15987.1.S1_at | Finished cDNA, clone ChEST481o6 | tcataccagtctcaagcaggg | tccttcattcctcactcaaaca |
12 | GgaAffx.11516.1.S1_s_at | similar to septin 2 | acttccgttcagagaggctg | agcacccgtccacttagaga |
13 | Gga.12446.1.S1_s_at | staufen, RNA binding protein (Drosophila) | ccgcagattgattttccttg | acttgcaaggctatcgtgct |