Figure 5

Hierarchical clustering of differentially expressed genes analyzed by quantitative RT-PCR. Based on the microarray analysis, the 23 genes selected as being up-regulated at stage X and the 13 genes selected as being up-regulated at HH stage 3 were confirmed by quantitative RT-PCR at stage X (0 h), HH stage 3 (12 h), HH stage 6 (24 h), and HH stage 9 (30 h). Most (91.7%, 33/36) of genes were consistent with results as shown of microarray data analysis. Color indicates the relative expression levels of each gene, with red indicating higher expression, green indicating negative expression. Black represents a control group that is normalized for the relative quantification of gene expression. Four genes with less than twofold expression increase at HH stage 3 were indicated (≸). NA is not annotated from NetAffx analysis tool.