Figure 2

Basal synaptic transmission is normal in the presence of CDK4 inhibitor. (A, B): Incubation in 5 μM CDK4 inhibitor(CDK4I) (n = 16 slices) for 30 min before recording had no effect on stimulus-response curves of fEPSP slope (mV/ms) vs. stimulus (mA) at the SC-CA1 synapses in hippocampal slices both from P8-15 and P21-35 mice(control, n= 12slices). Data are presented as mean ± standard error (SE). (C, D): plots of fEPSP slope (mV/ms) vs. presynaptic fiber volley amplitude (mV) from a random sample of slices from P8-15 and P21-35 animals. No differences were apparent between control (n = 20 slices) and CDK4I pretreated slices (n = 20 slices). (E, F)slices (n = 16 slices) were stimulated at a stimulus intensity that produced 50% of the maximal fEPSP for 15 min.CDK4I was then introduced into the perfusion buffer [artificial cerebrospinal fluid (ACSF)] at a concentration of 5 μM and responses were recorded for 1 h. No change in the fEPSP was observed both from P8-15 and P21-35 mice. Error bars correspond to SE.