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Figure 4 | BMC Developmental Biology

Figure 4

From: Plexin-B1 plays a redundant role during mouse development and in tumour angiogenesis

Figure 4

Generation of PlexinB1 mutant mice. A) Schematic structure of murine PlexinB1 gene, targeting construct and recombinant allele upon CRE deletion. In red is indicated the position of the probe for the Southern blot. B) Southern blot analysis of genomic DNA from recombinant ES cells after EcoRI digestion. A 15 Kb band in the WT and a 6.5 Kb band in the homologous recombinant (EcoRI sites are shown in A). C) The excision of exons 22 and 23 was checked using a primer pair that gives an amplicon of 800 bp upon CRE deletion and does not amplify the WT gene. D) RT PCR to reveal PlexinB1 mRNA was performed on total RNA from adult kidney. E) PlexinB1 protein in mutant mice is undetectable by IP and Western blot analysis of adult kidney lisates; the asterisk indicates a non specific band recognized by the antibody in the liver only.

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