Figure 5
From: The chemokine Sdf-1 and its receptor Cxcr4 are required for formation of muscle in zebrafish
Knockdown of E-box factors affects cxcr4a and sdf1a transcription. Dorsal views (A-F). Embryos between 13–14 h were analyzed. (A,D) Control showing cxcr4a (n = 20) and sdf1a (n = 20) expression respectively. (B,E) Double myoD-myf5 morphants demonstrate reduction of cxcr4a (n = 15/20) and sdf1a (n = 16/20) transcription respectively. This indicates cooperative function of MyoD and Myf5. (C,F) e12 morphants have vast reduction of cxcr4a (n = 20/20) and sdf1a (n = 20/20) transcription, this confirms that E12 is a major regulating factor. Black arrow in F indicates sdf1a staining in the non somitic lateral mesoderm. Abbreviation: n – notochord.