Figure 5

Regulation of survivin expression by vascular endothelial growth factor (VEGF) at 96 hpf. (a): Sub-intestinal vessels in uninjected Tg(fli1:EGFP)^y1 embryos. (b): Injection of human VEGF (2 ng) gave rise to ectopic angiogenesis (arrows). There was no observable ectopic angiogenesis in the ISV (c): Axial and inter-segmental vessels in untreated Tg(fli1:EGFP)^y1 embryos. (d): Tg(fli1:EGFP)^y1 embryos treated with VEGF tyrosine kinase receptor inhibitor (VEGFTKRI, 25 μmol/L) showing defective sprouting of inter-segmental vessels. (e): Injection of survivin mRNA did not reverse the defects seen in VEGFTKRI treated embryos. (f): Histogram showing the average survivin mRNA expression (expressed in fold-change) in untreated and VEGFTKRI treated embryos as well as in embryos injected with human VEGF. Results expressed in mean ± S.E.M. (n = 3 experiments using at least 20 embryos per experiments). When the three groups of data were compared using Kruskal-Wallis Test, p-value = 0.016. When the data of uninjected vs VEGFTKRI treated embryos were compared using Mann-Whitney U Test, p-value = 0.037.