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Figure 2 | BMC Developmental Biology

Figure 2

From: Silencing of core transcription factors in human EC cells highlights the importance of autocrine FGF signaling for self-renewal

Figure 2

Expression profiling of OCT4, NANOG, and SOX2 RNAi samples. (A): Overlaps of differentially expressed genes. The areas of the squares are proportional to the numbers of genes which they represent [77]. O = OCT4 knock-down, N = NANOG k.d., S = SOX2 k.d. (B): Correlation-based dendogram of RNAi samples and control. Note the high similarity between the OCT4 and SOX2 knock-downs. r = linear correlation coefficient. (C): Scatter plots of independent NANOG knock-downs and mock controls. esiRNA 1 and 2 were derived from non-overlapping parts of the NANOG mRNA. The dashed line indicates the expression threshold based upon negative control beads. Only significantly expressed genes were considered in the correlation analysis. (D): Array and real-time PCR-based mRNA expression measurements of NANOG downstream targets using the two independent NANOG esiRNA pools. Note the high degree of concordance between the independent knock-downs and methods of mRNA quantification. Values are means of two biological replicates ± SE.

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