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Figure 2 | BMC Developmental Biology

Figure 2

From: A Cre-lox approach for transient transgene expression in neural precursor cells and long-term tracking of their progeny in vitro and in vivo

Figure 2

Multipotency of postnatal mouse spinal cord NPCs grown as monolayers. A-D: Upon removal of growth factors and in the presence of FCS (1%), cells quickly differentiated into oligodendrocytes (A, O4, green), neurons (βIII-tubulin, red, B &D), and astrocytes (GFAP, red in A and green in B). C: Monolayer NPCs respond differently to change in differentiation conditions. Removal of FCS from the differentiation medium significantly increased the number of βIII-tubulin-positive cells from 0,59% ± 0,40 to 5,7% ± 3,2 at 48 hours in vitro. D: Confocal picture of βIII-tubulin positive cells showing long neurites after 7 days in differentiating medium. E-F: Neurosphere formation by monolayer NPCs cultures. E: Percentage of neurospheres formed after 2 weeks in clonal density NPC cultures (passage 4). Insert shows a typical example of the neurospheres formed from early passage (P4) monolayer NPCs cultures. F: Neurospheres generated by clonal density cultures of monolayer NPCs, differentiated into astrocytes (GFAP, green) and neurons (βIII-tubulin, red). The asterisk indicates the location of the neurosphere.

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