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Figure 1 | BMC Developmental Biology

Figure 1

From: A Cre-lox approach for transient transgene expression in neural precursor cells and long-term tracking of their progeny in vitro and in vivo

Figure 1

Isolation and maintenance of neural precursors cells (NPCs) from cre-reporter mice postnatal spinal cord as monolayer cultures. A: Image of live NPCs from mouse spinal cord monolayer cultures. Arrows show cells with large, flat and dark cell body with multiple processes. Arrow heads show fast proliferating cells with a small, spherical and bright cell body. B-D: Immunostaining analysis of NPC monolayer cultures. In the presence of mitotic factors (FGF-2 20 ng/ml & EGF 20 ng/ml), virtually all the cells co-expressed Nestin and Sox2 (red and green, respectively B) and none express either βIII-tubulin (C) or GFAP (D). E: Growth curves of spinal cord NPCs showed an identical exponential expansion at passage 6 and 28, with a population doubling time of ~24 h. F: Schematic protocol for the culture of NPCs from P5 mouse spinal cord as monolayers on polyornithine-laminin substrate.

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BMC Developmental Biology

ISSN: 1471-213X

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