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Table 5 Induction of sporulation in grlJ-

From: GrlJ, a Dictyostelium GABAB-like receptor with roles in post-aggregation development

Strains

SDF-1 in sorus

SDF-2 in sorus

Fold induction of spore formation

   

SDF-1

SDF-2

GABA

Glutamate

Wild-type*

103 U

5–20 103 U

3 ± 0.7

3.3 ± 0.2

3.3 ± 0.2

1.2 ± 0.3

grlJ -

103 U

104 U

3 ± 0.3

3.2 ± 0.4

3.2 ± 0.2

1.3 ± 0.2

  1. Culminants of each strain were harvested and dissociated in 1 ml cAMP buffer, washed once and counted. 105 cells were plated in 6 well plates in the presence of the indicated factors. The proportion of spores was determined under the microscope 2 h after induction and quantified as fold induction in comparison to uninduced cells. * Either A×2 or A×4: A×2 was initially used for induction by SDF-1 or SDF-2 [30]. A×4 is used for all factors and responds like A×2 to SDF-1 and SDF-2.