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Table 3 GrlJ mRNA quantification as determined by Real-Time RT-PCR (qRT-PCR)

From: GrlJ, a Dictyostelium GABAB-like receptor with roles in post-aggregation development

Time points (h)

GrlJ (pg)

GrlJ (relative levels)1

Actin control (pg)

0 h

4.0

1

502

4 h

2.8

0.85

412

8 h

3.6

1.18

382

12 h

9.9

2.30

539

16 h

10.8

2.25

605

20 h

11.1

2.85

487

  1. RNA was isolated from various developmental stages (given in hours). 1The relative levels of GrlJ during development were determined assuming that the level of the actin message is constant throughout development. The level of GrlJ at the 0 hour time point was arbitrarily set to 1.
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