Figure 9

(A) The grlJ^- strain shows alterations at the slug stage. For determination of the slug size, slugs were photographed with an inverted stereomicroscope and around 50 slugs per strain were marked with the DISKUS software program that allowed measuring uneven objects. p value, ≤ 0.005. (B) Slug migration during phototaxis. A×2 and grlJ^- cells were harvested and used for a phototaxis experiment. Both strains formed slugs phototaxing towards the light source. Figure Ia depicts the enlarged version of the box in I, which shows the path of the migrating A×2 slugs. IIa is an enlarged portion of the box in II which displays the pattern of slug movements in grlJ^-. As clearly indicated by the red arrows, the grlJ^- slug broke several times on its way. Bar, 1 cm.