Figure 8
From: GrlJ, a Dictyostelium GABAB-like receptor with roles in post-aggregation development
Expression of cell adhesion molecules in grlJ-. Protein samples were obtained at different time points during vegetative growth and development from A×2 cells and grlJ- mutants. The equivalent of 2 × 105 cells was loaded per lane. They were separated by SDS-PAGE and transferred to nitrocellulose using tank blotting. The blots were then probed with polyclonal antibodies specific for DdCAD-1 or gp-150 (LagC) [21,23]. csA was recognized by mAb 33-294-17 [22]. Whereas DdCAD-1 expression was not affected, csA and LagC were expressed earlier. For control the blots were probed for actin.