Figure 5
From: A role for sperm in regulation of egg-laying in the Nematode C. elegans
Detection of Extracellular MSP in the Uterus – Adult hermaphrodites were stained in whole-mount with monoclonal anti-MSP (red). DNA was detected with DAPI (blue). Bright red staining spermatozoa (arrows) are located near small red puncta and diffuse patches of staining, which likely correspond to extracellular MSP vesicles and free MSP, respectively [17]. While the patterns of sperm localization and MSP staining are slightly variable in the uterus, the staining observed in the egl-32(n155) and tm336 mutants was qualitatively within the normal range seen in the wild type. Sperm are approximately 4 μm in size.