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Figure 3 | BMC Developmental Biology

Figure 3

From: A role for sperm in regulation of egg-laying in the Nematode C. elegans

Figure 3

Rescue Experiments – (a) Cosmids from the region thought to be associated with egl-32 were injected into mutant egl-32(n155) animals. One cosmid from this interval, C26G6, which overlaps the sequenced cosmid T08G11, rescued the egl-32 phenotype. Among several subclones of this cosmid, one containing two predicted ORFs T08G11.2 and T08G11.3, but not one containing only T08G11.3, also rescued egl-32. (b) – Injection of the subclone along with rol-6 rescues the egg-laying defect of egl-32. * Statistically significant difference. Student t-test value < 0.05. (c) – Alignment of T08G11.2 with B0207.11 and F44F4.10. Identical amino acid residues are highlighted in black, similar amino acids in gray. The SH2-like domain is highlighted in yellow. Sequences deleted in deletion alleles are outlined in red. For the internal deletions in B0207.11 and F44F4.10 we have not determined whether an in-frame product is created.

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