Figure 8
From: Rybp, a polycomb complex-associated protein, is required for mouse eye development
Corneal neovascularization and irregular retinal development in ubiquitous Rybp transgenic mice. (A-F) Neovascularization of the ROSA26-RYBP/EGFP;β-Actin/Cre transgenic corneas in adult (2-month old) mice. Sagittal sections from wild type (A, B) and transgenic eyes (C, D) were stained with hematoxylin and eosin; boxes in panels A and C show areas magnified in panels B and D, respectively. The appearance of vessels (arrowheads in panel D) in the stroma of transgenic mice only is indicative of corneal neovascularization. The epithelium of the transgenic mice is also disorganized (D; open arrowhead). (E-F) Electron micrographs showing the corneal stroma next to the corneal epithelium in transgenic (Tg) and wild-type (Wt) animals at three weeks of age. While the stroma of the wild-type animal is not vascularized, a capillary (Ca) is seen close to the corneal epithelium in the transgenic animal. (G-H) Abnormal retinal folding and colobomas in newborn β-Actin/RYBP Tg mice (H) in comparison to normal retina in controls (G). C; cornea, Col; coloboma, E; epithelium, Ep; corneal epithelium; L; lens, NR; neuroretina, WT; wild type, S; stroma, TG; Transgenic. Magnification: A, C (×200); B, D (×460); G-H (×160)