Figure 5
From: Rybp, a polycomb complex-associated protein, is required for mouse eye development
Multiple ocular abnormalities in Rybp-/- <-> Rybp+/+ chimeric embryos. (A-B) Whole mount eyes from E13.5 wild type (A) and chimeric (B) embryos. The normal eye is symmetrical, while in the chimeric eye the chorioid fissure fails to close, leaving a large coloboma (arrowhead). (C-F) Histology of coronal sections of E13.5 wild type (C, E) and chimeric (D, F) eyes. Higher-magnification views of areas indicated in (C) and (D) are shown in (E) and (F), respectively. In the chimeric embryos, the eyes are rotated, lens development is delayed and overall lens size is reduced, and the retina is asymmetric with thickening on one side (F; arrowhead). (G-H) At E13.5 in the normal eye there is a mesenchymal cell layer between the SE and the LE (G; black arrowhead). In the chimeric eye, the lens epithelium is continuous and mixed with the mesenchyme and the surface epithelium (H; black arrowhead). H; hyaloid cavity, L; lens, LE; lens epithelium, NR; neuroretina, SE; surface ectoderm. Magnification: A-B (×120); C-D (×60); E-F (160×); G-H (×400)