Figure 1
From: The lin-35/ Rb and RNAi pathways cooperate to regulate a key cell cycle transition in C. elegans
Two classes of mutants with altered numbers of intestinal nuclei. (A) Shows a wild-type (upper panel), a class 1 mutant with fewer than the wild-type number of intestinal nuclei (rr44) (middle) and class 2 mutant with extra intestinal nuclei (lin-35(rr33)) (lower panel). All animals were at the L4 larval stage and express the intestinal-specific elt-2::GFP. Anterior is left in all pictures. (B) Genetic interaction between the class 1 and class 2 mutants. All mutants with less intestinal nuclei (class 1) are epistatic to the mutants with supernumerary nuclear divisions (class 2). The double mutant lin-35(rr33); rr45 is synthetic lethal, therefore it is not represented on this graph. (C) Lineage analysis of the intestinal cells in lin-35(rr33) mutants. The diagram is representative of observations collected from 8 independent larvae from the initiation of post-embryonic development up to the end of the L2 stage. Horizontal lines represent intestinal nuclear divisions, while the vertical lines represent time.