Figure 6

NOGO-A Expression During Network Establishment. A) H&E and B) NOGO-A in situ hybridization (ISH) of HH36 (E10) optic tectum, prior to synaptogenesis. Transcript expression is intensified in cells of the tectal associated nuclei (TN) and cortical neuronal lamina. Note the lack of expression in the fibers of the optic tract (OT). C&D) Magnified view of tectal lamination (horizontal boxes) from A&B reveal the presumptive projection layer of the tectum, the compartment stratum griseum central (SGC), as the cortical neuronal lamina strongly expressing NOGO-A. Ventricular Zone-VZ, developing lamina-DL. E) H&E and F) NOGO-A in situ hybridization at E20 (after significant synaptogenesis) shows punctate expression localized to the large neuronal cell bodies of the tectal associated nuclei, the SGC, and to deep cellular layers of the main retinal afferent layer, the stratum griseum et fibrosum superficiale (SGFS). G&H) Magnified view of tectal lamination (horizontal boxes) from C&D. I-M) Serial sections localizing NOGO-A expression to projection neurons within a tectal associated nucleus (vertical box in C). I) H&E showing the large cell bodies of the projection neurons (inset showing large cytoplasm-rich neuron with nuclear clearing and prominent nucleolus), J) punctate NOGO-A expression, K) immunohistochemistry (IHC) of neurofilament (NF), highlighting neuronal cell bodies, L) IHC of glial fiber associated protein (GFAP) demonstrating glial supporting cells, and M) IHC for vimentin (Vim) demarcating a few immature glial cells.