Large-scale RNAi screens identify novel genes that interact with the C. elegans retinoblastoma pathway as well as splicing-related components with synMuv B activity

Table 3 Summary of RNAi phenotypes of splicing related synMuv genes in synMuv B activity, transgene silencing (scm::GFP), ectopic expression of lag-2::GFP reporter, extra intestinal cells (elt-2::GFP), ectopic P granules, and rescue of cyd-1.

	*lin-35*	*rsr-2*	*lsm-2*	*lsm-4*	*snr's*(1–7)
RNAi phenotype in WT	Rbs	Ste, Lva	WT	WT	Emb, Lva, Lvl
synMuv B	+++	+	+	+	+
synMuv B (25°C)	+++	n/a	++	++	n/a
Transgene silencing (25°C)	++	-	-	-	n/a
Ectopic *lag-2::GFP*	+	-	-	-	+
Ectopic *lag-2::GFP* (25°C)	++	-	++	++	+
Extra intestinal cells	+	+	-	-	-
Ectopic P granules	+++	-	-	-	+
*cyd-1* rescue	+	-	-	-	-

Summary of RNAi phenotypes of splicing related genes: synMuv B activity, transgene silencing (scm::GFP), ectopic expression of lag-2::GFP reporter, extra intestinal cells (elt-2::GFP), ectopic P granules, and rescue of cyd-1. If 25°C is not indicated, experiments were performed at 20°C. “+++”, “++”, “+” and “–“stand for strong, moderate, low and no effect respectively. (n/a: not annotated).

ISSN: 1471-213X