Table 2 Novel synMuv B genes and genetic epistasis analysis.
% of animals with 2, 3, 4 or 5 pseudovulvae | ||||||
|---|---|---|---|---|---|---|
2 | 3 | 4 | 5 | % of Muv | n | |
synMuv B activity | ||||||
rsr-2(RNAi) | 0% | 0% | 0% | 0% | 0% | 445 |
rsr-2(RNAi); lin-15A(n767) | 8.6% | 1.3% | 0% | 0% | 10% | 453 |
rsr-2(RNAi); lin-8(n111) | 1.5% | 0% | 0% | 0% | 2% | 197 |
rsr-2(RNAi); lin-38(n751) | 1.0% | 0.1% | 0% | 0% | 1% | 787 |
rsr-2(RNAi); lin-56(n2728) | 2.0% | 0% | 0% | 0% | 2% | 595 |
rrf-3(pk1426); lsm-2(RNAi) | 0% | 0% | 0% | 0% | 0% | >300 |
lsm-2(RNAi); lin-15A(n767) | 1.5% | 3.1% | 1.0% | 0% | 6% | 390 |
lsm-2(RNAi) 25°C | 0% | 0% | 0% | 0% | 0% | 220 |
lsm-2(RNAi); lin-15A(n767) 25°C | 13.8% | 42.5% | 37.8% | 0.5% | 94% | 188 |
lsm-2(RNAi); lin-8(n111) 25°C | 14.7% | 9.5% | 1.0% | 1.0% | 26% | 136 |
lsm-2(RNAi); lin-38(n751) 25°C | 13.4% | 27.4% | 17.8% | 0% | 60% | 157 |
lsm-2(RNAi); lin-56(n2728) 25°C | 7.1% | 8.2% | 4.7% | 0% | 20% | 85 |
rrf-3(pk1426); lsm-4(RNAi) | 0% | 0% | 0% | 0% | 0% | >300 |
lsm-4(RNAi); lin-15A(n767) | 2.4% | 5.0% | 0.5% | 0% | 8% | 420 |
lsm-4(RNAi) 25°C | 0% | 0% | 0% | 0% | 0% | 149 |
lsm-4(RNAi); lin-15A(n767) 25°C | 16.0% | 40.8% | 38.3% | 0.6% | 95% | 162 |
lsm-4(RNAi); lin-8(n111) 25°C | 11.7% | 9.0% | 1.4% | 0% | 22% | 145 |
lsm-4(RNAi); lin-38(n751) 25°C | 10.5% | 45.3% | 23.2% | 0% | 79% | 95 |
lsm-4(RNAi); lin-56(n2728) 25°C | 13.2% | 14.0% | 5.3% | 0% | 33% | 114 |
lin-37(RNAi); lin-15A(n767) | 27.6% | 41.4% | 24.1% | 6.9% | 100% | 29 |
lin-35(RNAi); lin-15A(n767) | 0% | 27.5% | 62.5% | 10.0% | 100% | 40 |
lin-35(RNAi); lin-38(n751) | 1.6% | 1.6% | 67.2% | 22.9% | 93% | 61 |
lin-35(RNAi); lin-15A(n767) 25°C | 0% | 0% | 33.7% | 58.7% | 92% | 92 |
lin-35(RNAi); lin-8(n111) 25°C | 1.2% | 7.1% | 29.4% | 0% | 37% | 85 |
lin-35(RNAi); lin-56(n2728) 25°C | 1.2% | 6.1% | 37.8% | 45.1% | 89% | 82 |
snr-1(RNAi)(L1)(MV) | 0% | 0% | 0% | 0% | 0% | >200 |
lin-35(n2239); snr-1(RNAi)(L1)(MV) | 0% | 0% | 0% | 0% | 0% | >200 |
snr-1(RNAi)(L1)(MV); lin-15A(n767) | 9.3% | 3.6% | 4.3% | 0.4% | 18% | 278 |
snr-1(RNAi)(L1)(JA); lin-15A(n767) | 1.6% | 3.2% | 0% | 0% | 5% | 62 |
snr-1(RNAi)(L1)(MV); lin-56(n2728) | 2.4% | 1.4% | 0.7% | 0% | 5% | 293 |
snr-1(RNAi)(L1)(MV); lin-38(n751) | 3.3% | 1.1% | 0% | 0% | 4% | 132 |
snr-2(RNAi)(L1)(MV); lin-15A(n767) | 1.0% | 1.0% | 0% | 0% | 2% | 93 |
snr-3(RNAi)(L1)(MV) | 0% | 0% | 0% | 0% | 0% | >200 |
lin-35(n2239); snr-3(RNAi)(L1)(MV) | 0% | 0% | 0% | 0% | 0% | >200 |
snr-3(RNAi)(L1)(MV); lin-15A(n767) | 14.0% | 4.0% | 0.5% | 0% | 19% | 221 |
snr-3(RNAi)(L1)(MV); lin-38(n751) | 2.4% | 0.8% | 0% | 0% | 3% | 126 |
snr-4(RNAi)(L1)(MV); lin-15A(n767) | 9.7% | 4.8% | 1.2% | 0% | 16% | 83 |
snr-4(RNAi)(L1)(JA); lin-15A(n767) | 7.1% | 3.2% | 0% | 0% | 10% | 156 |
snr-5(RNAi)(L1)(MV); lin-15A(n767) | 1.3% | 1.3% | 0.6% | 0% | 3% | 158 |
snr-6(RNAi)(L1)(MV); lin-15A(n767) | 4.7% | 3.5% | 0% | 0% | 8% | 257 |
snr-7(RNAi)(L1)(MV); lin-15A(n767) | 10.7% | 7.1% | 2.2% | 0% | 20% | 140 |
EGF/RAS/MAPK epistasis | ||||||
rsr-2(RNAi); let-23(sy97); lin-15A(n767) | 0% | 0% | 0% | 0% | 0% | 99 |
rsr-2(RNAi); let-60(n1876); lin-15A(n767) | 0% | 0% | 0% | 0% | 0% | 43 |
rsr-2(RNAi); lin-3(n378); lin-15A(n767) | 5.0% | 1.3% | 0% | 0% | 6% | 80 |
let-60(n1876); lsm-2(RNAi); lin-15A(n767) | 0% | 0% | 0% | 0% | 0% | 34 |
lin-3(n378); lsm-2(RNAi); lin-15A(n767) | 1.6% | 0% | 0% | 0% | 2% | 250 |
lsm-4(RNAi); let-60(n1876); lin-15A(n767) | 0% | 0% | 0% | 0% | 0% | 64 |
lsm-4(RNAi); lin-3(n378); lin-15A(n767) | 2.7% | 0.6% | 0% | 0% | 3% | 180 |
lin-35(RNAi); let-23(sy97); lin-15A(n767) | 0% | 0% | 0% | 0% | 0% | 58 |
lin-35(RNAi); let-60(n1876); lin-15A(n767) | 0% | 0% | 0% | 0% | 0% | 25 |
lin-35(RNAi); lin-3(n378); lin-15A(n767) | 3.2% | 15.9% | 74.6% | 6.3% | 100% | 63 |