| | | N2 |
rrf-3 (pk1426)
|
lin-35 (n2239)
|
lin-37 (n758)
|
zfp-2 (tm557)
|
|---|
RNAi | Phenotype | | | | | |
|---|
gpc-2
| Emb | 0% | 14.9% | 2.6% | 2.8% | 0% |
mom-2
| Emb | 17.8% | 40.1% | 64.1% | 67.4% | 69.4% |
hmr-1
| Lvl | 0% | Ste, Ooc, Emb | 80.7% | 77.6% | 53.1% |
cel-1
| Lva | 0.5% | 100% | 98.6% | 11%* | 23.2% |
unc-87
| Unc | 3.6% | 0% | 94.2% | 85.3% | 0% |
unc-15
| Unc | 0% | 100% | 96.5% | 92.6% | 0% |
- We tested several RNAi clones, previously used by others to quantitatively examine sensitivity to RNAi, in different genetic backgrounds [26,62,63]. To allow comparison with RNAi effects reported by others, we used RNAi clones from the Ahringer Library [64] except for cel-1(Vidal library). Numbers are averages of 3 to 6 experiments and indicate proportion of animals (F1 progeny) displaying the corresponding phenotype. In agreement with recent findings [26,65], we also observed that different RNAi hypersensitive mutants showed surprising gene specificity in RNAi enhancement.
- *cel-1 RNAi in lin-37(n758) produces larval arrest at later stages
