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Figure 4 | BMC Developmental Biology

Figure 4

From: Perlecan controls neurogenesis in the developing telencephalon

Figure 4

Defective neurogenesis in the pallium of perlecan-deficient embryos. (A, B) Double immunofluorescence for Ki67 (red) and BrdU (green) after 24 hours survival to a E12.5 BrdU pulse was used to calculate the fraction of cells exiting the cell cycle (quitting fraction) at E13.5. Cells labeled only with BrdU, a pool no longer dividing, are abundant in the cortical plate in the wild-type embryos (CP in A), but its number in the perlecan-null neocortex decreases considerably (B). (C) Quantification of the fraction of cells leaving the mitotic cycle (quitting fraction: percentage of the number of BrdU+, Ki67- cells among the total of BrdU+ cells) in two E13.5 litters. In the perlecan mutants, the number of cells that leave the cell cycle is reduced to approximately a half of that in wild-type littermates, n = 6 slices.* p < 0.05 and ** p < 0.01. (D, E) Double labeling with phosphorylated histone H3 (red) and BrdU (green) after 2 hours survival to a BrdU pulse, used to compare G2/M phase in wild-type (D) and perlecan-null (E) neocortex at E16.5. (F) Quantification of the percentage of cells in G2/M cell cycle phase (double labeled cells; arrows in D) referred to the total of BrdU labeled cells. n = 12 slices. Scale bar: 40 μm (A, B, D, E).

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