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Figure 2 | BMC Developmental Biology

Figure 2

From: Perlecan controls neurogenesis in the developing telencephalon

Figure 2

Cell proliferation decays in the absence of perlecan. (A, B) Mitotic cells in M phase immunodetected with an anti-phospho-histone H3 antibody in the telencephalic vesicles of E13.5 wild-type (A) and perlecan-null (B) embryos. The neocortex shows two well-differentiated mitotic populations: the ventricular primary population in the VZ, and the basal progenitors in the SVZ that emerges in the middle of the pallium at E13.5. (C) The mitotic index (the percentage of phospho-histone H3-labeled cells among Ki67+ progenitors) shows significant differences in the MGE, but not in the neocortex at E13.5. At E16.5, the mitotic index in the neocortical primordium is reduced to about a 60% of that in wild-type embryos, affecting both the VZ and the SVZ mitotic populations. Values are the mean ± SEM, n = 5 at E13.5; n = 4 at E16.5. * p < 0.05; ** p < 0.001. (D, E) TUNEL staining of E15.5 wild-type (D) and perlecan-null (E) dorsal neocortex. As in the wild-type littermates, very few TUNEL-positive cells (arrows in D and E) were observed in perlecan-null embryos. Scale bars: 150 μm (A, B), 80 μm (D, E).

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